Thioacetamide (TAA) is a thiono-sulfur containing compound with a wide manufacturing application. Humans and animals exposure to TAA may occur in different ways and may cause nephrotoxicity. So, in this study serum creatinine concentration and urea, in addition to renal pathological changes, were examined in mice treated with TAA/P (100 mg/kg B.W). One hundred twenty male albino (BALB/c) mice were used. They were randomly divided into 2 main groups. In the control group 30 mice were fed water only and normal mice pellet.The other TAA-treated group of mice were divided into 3 subgroups as follow: 1^st group (G1) was injectsed with TAA for 2 months (injected twice a month), while the 2^nd(G2) and 3^rd(G3) groups were injected with TAA for 4 and 6 months respectively (single injection monthly). Subsequently, serum urea and creatinine were measured in the control and the treated mice. The pathological changes in renal tissue were studied by examiningstained Hematoxylin and eosin (H and E). The results showed a significant increase (P<0.05) in blood urea and creatinine levels of all studied groups with diverse pathological changes in kidney, including degeneration, necrosis, hemorrhage, nephritis, abscess and granulomatous lesions due to TAA toxicity.