Probiotics mean live microorganisms that have beneficial effects on their host’s health. The purpose of this study was to compare aqueous, methanolic of some traditional plant extracts on the viability of dietary probiotic supplementation [a dietary probiotic (Protexin)]. Also, this study was conducted to evaluate the antibacterial effect of various extracts concentrations from 1.25 to 100 mg/ml of Rosmarinus officinal, Glycyrchiza glabra, Hibiscus sabdriffo, Curcuma longa, Citrus auratifolia swingle, Cinnamomum zeylancium, Urtica dioica, Thymus vulgaris, Punica grantum and Zingiber officinal on activities of probiotic bacteria Lactobacillus, Bifidobacterium, Streptococcus thermophillus by determining the minimum inhibitory concentrations (MICs). Complete Randomized Design (CRD) was used as experimental design. Means were compared according to L.S.D. values at 5% significant level. The result appeared that methanolic extract gave high significant effect on bacteria compared with aquatic extracts which may be due to the highly acidity. Depending on phytochemical screening, the highest concentration of the total identified phenols and Flavonoids were (35.63 and 13.53 %) for R. officinus methanolic extracts. Based on the results of this study R. officinus and C. auratifolia methanolic extracts had the lowest MIC 6.25 mg/ml against probiotic bacteria than the other tested extracts. Taken together, evidence gathered in the present study indicates that   Z. Officinale, G. glabra, T. vulgaris and U. dioica aquatics extracts can be more safely in treatment of digestive diseases. More study most conducted to evaluate the pH for the traditional plants extracts to guarantee their useful uses in treatment of digestive diseases.