Although Bacteroides fragilis is a bacterium present within gut microbiota, the toxin producer strain, known as enterotoxigenic B. fragilis (ETBF), is associated with diarrhea in children less than 5 years of age. This study includes 69 diarrheal and 29 non-diarrheal (control) samples collected from children less than 5 years old. DNA was extracted directly from stool specimens and directed to conventional PCR targeting beta-isopropylmalate dehydrogenase (leuB) gene, used for detection of B. fragilis, and Bacteroides fragilis toxin (bft) gene, used for the detection of ETBF. The results showed that the prevalence of leuB gene was 78 (79.6%) including 56 (81.2%) in diarrheal and 22 (75.9%) in non-diarrheal subjects, while that of bft gene was only 3 (3.1%) including 2 (2.9%) in diarrheal and 1 (3.4%) in non-diarrheal subjects. Based on sequencing of bft-positive specimens, both bft-1 and bft-2 isoforms were represented in diarrheal specimens, whereas only bft-1 was found in the control specimens. In conclusion, this study examined for the first time the leuB and bft gene in a specimen of Iraqi children with diarrhea and showed no significant differences between diarrheal and control groups in both genes.
Back ground: Meningitis is an inflammation of the protective membranes covering the brain and spinal cord that known as meninges. It is the major cause of morbidity and mortality among infants and children less than the age of 5 years.
Objectives: To determine Haemophilus influenzae type b meningitis incidence in children aged less than 5 years and choice a drug for treatment
Patients and methods: During period from Febraury 2012 to first March 2013 ,250 Cerebrospinal fluid (CSF) specimens were collected from children less than 5 years ,which clinically diagnosed with meningitis ,in several hospitals of Iraq.Microbiological, biochemical, latex agglutination test and antibiotics sensitivity were used to identify and choice the antib
Background: Non-nutritive sucking habits are common in infants and toddlers. One of the most common non-nutritive sucking habits is pacifier; its prevalence varies from one population to another. This study was conducted to determine the prevalence of pacifier sucking habit among children aged 1-5 years old in Baghdad city and to assess its effect on the occlusion of primary dentition concerning posterior crossbite. Materials and methods: The study was carried out among 1222 children aged 1-5 years old, from which 50 children with continues pacifier sucking habit were chosen to be the study group, compared to 50 children without any sucking habit (control group) matching the study group in age and gender. Children were examined clinically t
... Show MoreBackground: Acute renal failure (ARF) in children is a catastrophic, life -threatening event
Aim of the study: the aim of the present study is to find out the etiology and outcome of patients with acute renal failure below two years of age admitted to the hospital.
Patients and methods: A prospective study was carried out on children below 2 years of age (with exclusion of neonatal period) presented with acute renal shut down
admitted to Children Welfare Teaching Hospital during the period from Jan. 1st, 2007tojan30th,2008.
Results: the study group included 100 patients with acute renal failure, 69 males and 31 females with M/F ratio 2.2:1.Age range was between (2 months - 2 years).
Prerenal ARF wa
Background: streptococcal pneumonia is one of the most common pathogens that cause otolaryngological disease.
Enzyme activity were studied in the sera of children with leukemia than healthy children, where 31 cases were studied, including 21 cases of patients with acute lymphatic leukemia
Objective(s): The aim of this study was to assess the severity of anemia among children under 5 years and to find
out relationship between severity of anemia in children with socio-demographic variables such as: child gender,
age, supplement diet, hookworm, pica, parent's education level, father's and mother's occupation, and family
monthly income with severity of anemia
Methodology: A descriptive cross sectional study was conducted at the primary health care centers in Alsadr city
for the period of 1
st July 2011 to the 1st November 2011.Non probability (purposive) sample of (630) children who
aged under 5 years and who attended to primary health care centers of Alsadr city. The data were collected
through using
ObjectiveIs to study the causes of bloody diarrhea in in (50)cases (37%) , Shigella spp in (25) cases (18% and
children under five years of age and to clarify their relations Campylobacter jejuni Icases only (0.75%)
to the type offood and mothers educational level ConciusionBloody diarrehea is common in children under
methods Ahospital based study was carried out at tikreet 5years age who were admitted to Tikreet Teaching Hospital
teaching Hospital in Tikreet city on 133 children who were in Tikreet city , There is astrong positive relationship
admitted to the hospital with bloody diarrehea their ages between the occurrence of bloody diarrehea and the type of
range between one month -5years. The period study is from foo
Background: The periodic review conducted of acute bacterial meningitis in children younger than 5 years of age in Baghdad to reflect changes in the epidemiological pattern of this infection.
Patients and Methods: Sixty patients with signs and symptoms suggestive of meningitis who were admitted to Central Teaching Hospital in Baghdad City were included in a prospective study started from the 1st October till the 30th of December 2009. By bacteriological study, biochemical and cytological study in the laboratory, WBCs in the blood with differential count was done to support the diagnosis of bacterial meningitis.
Results: Present study revealed that from (60) patients (6) cases (10.0 %) showed posi
Avery large numbers of articles are made by powder metallurgical methods using electrolytically reduced metal powders. Iron powder is one of these powders which play an important role in this field. Its preparation by electrolytic method is economic in comparison with the traditional methods (Atomization and carbonyl processes).
An electrochemical cell consisting of two electrodes (stainless steel cathode and iron anode, 99.9%) was used to study the electrolytic preparation of iron powder with particle size less than 106µm directly as powde1y form. Ferrous sulphate electrolyte was used containing sodium chloride as a stabilizing agent. The produced powder was thoroughly washed with an acidified distilled water and absolute ethan
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