Extracellular alkaline protease production by Aspergillus niger was studied with
the presence of different carbon and nitrogen sources availability in batch culture
condition .A. niger showed better protease production in the presence of organic
nitrogen sources compare to inorganic nitrogen sources in the medium, which
consist of 1% glucose as carbon source, 1.5% casein hydrolysate as nitrogen source
and 1% Nacl at initial pH=10 on rotary shaker incubator 140 rpm at 40 ºC for 5
days.