The output argon plasma stream was generated, and its properties were studied. The deionized water was formed using the optically described plasma stream. The titanium plate was melted using a needle with the argon gas stream. An analysis was conducted on the emission spectra of the plasma generated within the argon gas plasma jet system at different operating voltages, a flow rate of 4 L/min, and the pressure of the atmosphere. The amounts of NO2, NO3, and H2O2 that the lab will treat to evaluate their impact on removing bacterial aggregation were determined by measuring the absorbance of the plasma-treated water at wavelengths between 300 and 400 nm. Using optical emission spectrometry (OES), we measured the electron temperature, Debye length, and Debye range while examining the excited titanium metal's spectral lines. A series of spectra generated by the plasma were recorded under different flow rates of argon gas. The temperature and electron band density (Te) and (ne) of titanium metal and argon gas increased with increasing the flow rate of argon gas into the DC plasma, and increased killing of bacteria was observed with increasing the potential difference of ionized water in the plasma. This study shows that prepared cold plasma has beneficial effects on killing bacteria.