In this study, nickel oxide nanoparticles (NiO-NPs) were synthesized using 
nickel nitrate hexahydrate and licorice leaves extract, which served as both a 
reducing agent and a capping agent. The optical, structural, and microscopic 
properties of the NiO-NPs were characterized using a range of techniques, including 
UV-visible spectroscopy, Fourier transform infrared spectroscopy, energy
dispersive X-ray analysis, field emission scanning electron microscopy, and atomic 
force microscopy. The bio-reducing potential of Licorice leaves was examined 
using gas chromatography-mass spectroscopy for phytochemical analysis of plant 
extract. Furthermore, cell cytotoxicity investigation was conducted on the Mg-63 
cell line derived from osteosarcoma by the MTT method .The results showed that 
the viability of cancer cells was reduced with increasing NiO NPs concentration up 
to 28.78% for MG-63 Cells at the maximum concentration (500 μg/ml) in 
comparison with 80.79 % for HFF cell viability as control cells, while the 
antimicrobial experiments have demonstrated that NiO–NP action of two types of 
gram-positive bacteria and for two types of gram-negative bacteria. The results 
showed that NiO NPs had the highest antibacterial activity at high concentrations 
(1024 ug/ mL), forming the largest inhibition zone against Escherichia coli, 
Pseudomonas aeruginosa, Staphylococci aureus and Streptococcus with inhibition 
zones 18mm,18mm ,15mm and 22 mm respectively.