In the present study, the aim was made to identify the relationship between thyroid autoimmunity (TAI) and female infertility. The study was performed on 30 infertile women and 22 age-matched healthy fertile control age (33 ± 5 years). Overall, serum prolactin (PRL), thyroid stimulating hormone (TSH) assay is the key test for the diagnosis and management of hypo and hyperthyroidism. Anti-TPO Ab and anti-TG Ab were measured. The mean ± SE of serum PRL (31.080 ± 3.06) ng/ml was significantly (P<0.05) higher in infertile group compared with control (16.191±1.36) ng/ml. Serum TSH was significantly (P<0.05) higher in infertile group (5.689 ± 1.12) μIU/ml compared to control group (2.282 ± 0.18) μIU/ml. The prevalence of positive thyroid peroxidase antibody (TPO-Ab) was higher infertile women (248.439 ± 88.77) IU/ml than that of the control (15.118 ± 2.75) IU/ml. Also there was significant differences (P<0.05) in Anti-TG Ab titration in infertile women (360.139 ± 210.32) IU/ml compared to control (31.636±4.69) IU/ml. However, when thyroid antibodies were positive, hypothyroidism was more frequent in infertile women as compared to the control group. In the other hand, a significant (P<0.01) positive correlation between TSH hormone and Anti TPO Ab r = 0.37, while non-significant correlation was found between Anti- TG Ab and TSH. The present study demonstrated that autoimmune thyroiditis in infertile women is higher than that in healthy fertile controls.