Biomedical alloy 316L stainless steel enhancing to replace biological tissue or to help stabilize a biological structure, such as bone tissue, enhancing were coated with deposition a thin layer of silver nanoparticles as anti-bacterial materials by using DC- magnetron sputtering device. The morphology surface of The growth nanostructure under the influence of different working pressure were studied by atomic force microscope. The average grain size decrease but roughness of the silver thin layer was increased with‖ ―increasing the working pressure. The thickness of silver thin layer was increased from 107 nm at 0.08 mbar to 126 nm at 1.1 mbar. Antimicrobial activity of silver thin layers at different working pressure were studied. Th

viruses are responsible for a large proportion of lower respiratory tract infections (LRTIs). Other causes of LRTIs are bacteria: Streptococcus pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, and Staphylococcus aureus being the most common. Sputum samples are commonly used in the microbiological laboratory for diagnosing lower respiratory infections. Objective: The aim of this study to evaluate the causative bacteria and antibiotics sensitivity in culture of sputum samples. Patients Methods: A retrospective study performed in the microbiology department of Al Immamin Al Kahdimin Medical laboratory in Baghdad. The results of sputum cultures collected from the files between 2016 and 2019. A tota

Introduction and Aim: Klebsiella pneumoniae is a Gram-negative bacterium responsible for a wide range of infections, including respiratory tract infections (RTIs). This research was aimed to study the antibacterial and anti-biofilm effect of AgNPs produced by Gram positive and negative bacteria on RTIs associated with K. pneumoniae.   Materials and Methods: The biofilm formation of K.  pneumoniae was determined by tube method qualitatively from select bacterial species characterized by UV-Visible spectroscopy. The antibacterial susceptibility of the bacteria AgNPs was tested for their antibacterial and antibiofilm activity on a clinical isolate of K. pneumoniae.   Results: K. pneumoniae isolated from RTIs were strong biofilm prod

The aim of the present study was assess the antimicrobial effect of
Peganumharmala L seeds extracts by ethanol (80%) on gram negative and gram
positive bacteria and four concentrations (25, 50, 75 and 100) mg/ml were prepared.
Four clinical isolates of bacteria were used; two were positive and two were
negative bacteria; that include: Bacillus, Staphylococcus aureus, Pseudomonas
aeruginosa and Escherichia coli. The results showed that all concentration that have
been used had antimicrobial effect against gram negative and gram positive bacteria
and the best concentration that have the best antimicrobial effect was 100 mg/ml and
the effect of alcoholic extraction was greater on gram positive bacteria than gram
n

         The study included evaluation of cell surface charge and hydrophobicity of Escherichia coli, Klebsilla aerogenes, Proteus spp, Bacillus cereus, Staphylococcus epidermidis, Staphylococcus aureus(1) and Staphylococcus aureus(2) were determined by hydrocarbon adherence and hydrophobic interaction chromatography. The results showed that the negative charge of cell surface of gram negative bacteria was much higher than on gram positive once when these bacteria were grown on nutrient agar at 37 c for 18 h . E.coli was more negative charged than Klebsilla aerogenes and Proteus spp. The hydrophobicity of gram positive bacteria was much

he development of multidrug resistance in Gram-negative bacteria is a major problem faced antimicrobial therapy and management of infectious diseases. Too many resistance mechanisms were developed since the antimicrobial agents were produced and actually used. The mechanisms involved in antimicrobial agents' resistance are, modifying enzymes, alteration of the target site of antimicrobials and prevention of antimicrobials accumulation inside the bacterial cells. The latest is accomplished by two mechanisms: alteration of outer membrane permeability and efflux pumps. Efflux pumps are either chromosomal or plasmid-encoded although chromosomal encoded efflux pumps are common in Gram-negative bacteria. Resistance nodulation division (RND) efflu

The antibacterial effect of (Eruca sativa) extract was evaluated by an in vitro study testing the growth of various Gram-Positive and Gram-Negative bacteria . The bactericidal activity of this extract was analyzed by serial dilution in tubes. This study,found that Gram-Negative and Gram-Positive bacteria susceptible to very low eruca concentrations. On the other hand, Gram-positive bacteria were more susceptible than Gram-negative bacteria, the minimal bactericidal concentration of Gram-positive bacteria was 5 mg ml-1 but minimal bactericidal concentration of Gram-negative bacteria was 10 mg ml-1 that mean duble inhibation concentration of Gram-positive bacteria . this study suggest that Eruca sativa leaves have inhibation effect on Gra

Background: Nosocomial respiratory infections in the intensive care is one of the challenging issues, competing with other major causes of morbidity and mortality, that’s why it needs to be studied thoroughly.

Objectives: To assess the prevalence of colonization of respiratory tract by Gram-negative rods (GNRs) for critically ill patients in Baghdad teaching  hospital, and to determine antimicrobial resistance of the isolated strains.

Patients and Methods: A cross sectional study conducted on critically patients at the ICU/Baghdad teaching hospital during the period from December 2012-june 2013. thirty four  adult patients on mechanical ventilator  with e

Background:

Titanium dioxide TiO2 has been widely utilized in cleaning and sterilizing material for many clinical tools sanitary ware, food tableware and cooking and items for use in hospitals. Titanium dioxide TiO2 non toxicity and long term physical and chemical stability. It has been widely used decomposition of organic compounds and microbial organisms such as cancer cell, viruses and bacteria as well as its potential application in sterilization of medical devices. The aim of the study the effect of titanium dioxide TiO2 on some Gram negative bacteria and study their effects on some virulence factors and chromosomal DNA.In this study, we obtained (E. coli ? Proteus mirabilis, Proteus vulgaris ? Pseudomonas aeruginosa ? Klebsiella pneumonia and Ac