IIn this work, carboxymethyl cellulose/polyanionic cellulose (CMC HV/PAC LV) films were prepared and exposed to different X-ray doses (0, 200, 400, 600 and 800 cGy). Absorption and transmission measurements of the ultraviolet spectrum of the incident light were studied with a DU 800 spectrophotometer. Stress and strain were calculated by applying a tensile force to the samples and increasing its amplitude until the sample breaks. Applying this force to the samples increased the length of the samples. The absorption of CMC HV/PAC LV films increased with increasing X-ray dose, and therefore, the absorption coefficient increased with increasing X-ray dose. The results show that the amount of spectral transmittance of the films decreased with increasing X-ray dose, indicating that the opacity of the samples increased with increasing X-ray dose. The characteristic absorption peaks are observed at 275 nm^-1, 328 nm^-1, 345 nm^-1, 370 nm^-1, and 470 nm^-1, indicating molecular bonding. Increasing the X-ray dose decreases the elastic modulus of the films, and the stress amplification factor decreases with increasing strain ratio, indicating that X-ray photon irradiation leads to a decrease in the elasticity of the films. The results of this research may be useful in many fields, including industrial, medical or scientific research, as well as in oil fields.