Sediment accumulated in sewers is a major concern source as it induces numerous operational and environmental problems. For instance, during wet weather flow, the re-suspension of this sediment accompanied by the combined sewer overflow may cause huge pollutant load to the receiving water body. The characteristics of the sewer sediment are important as it shapes its behaviour and determines the extent of the pollution load. In this paper, an investigation of sewer sediment and its characterization is done for a case study in Baghdad city. Sediment depth covers more than 50% of the sewer cross-sectional area; several operational causes are comprised to cause this huge depths of sediment depositions. The testing and analysis of the sediment showed that the median particle size of the sediment is 0.3 mm, which infer a poorly graded sandy sediment in accordance with the unified classification system, particle’s specific gravity to be 2.63; with a water content of 41%. The organic content is tested and found to be 32.45 g per kg of sediment (equivalent to 3.24%).