Article - Extraction, Identification and Quantification of Vitexin and p-Coumaric Acid from Basella alba L. Cultivated in Iraq - Digital Repository

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Publication Date

Wed Jun 24 2026

Journal Name

Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)

Volume

35

Issue Number

2

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Extraction, Identification and Quantification of Vitexin and p-Coumaric Acid from Basella alba L. Cultivated in Iraq

Sara H. Hachim

Nabaa M. Ibrahem

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The objective of this work is to isolate, characterize, and quantify two bioactive substances from Basella alba (Malabar spinach), a plant of nutritional and therapeutic value. Vitexin and p-coumaric acid were isolated and quantified using high-performance liquid chromatography (HPLC), and Their characterization was carried out using Fourier transform infrared spectroscopy (FT-IR), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the spiking method, where the isolated compound was compared with the standard for confirmation. confirmationThe concentrations of p-coumaric acid and vitexin in Basella alba were found to be 144 µg/g and 220 µg/g, respectively, which are higher than those identified in previous studies. This suggests that environmental factors, particularly light exposure and geographical conditions, may play a significant role in enhancing the production of these secondary metabolites. Soxhlet extraction was employed to isolate and quantify the compounds, demonstrating high recovery rates consistent with previous findings.. These results highlight the potential of Basella alba as a valuable plant for further research into its health benefits. Future studies should explore its applications in nutraceuticals and pharmaceuticals.

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