Pulse wave speed (c) is a key variable in haemodynamics and cardiovascular system analysis. It is directly related to arterial elasticity and inversely to distensibility of the arterial lumen. The pressure–velocity loop (PU-loop), derived from simultaneous velocity (U) and pressure (P) measurements, is used to estimate c, leveraging the linear U–P relationship during early systole, when reflected waves are absent due to bifurcations and/or occlusion. The PU-loop method was validated using tensile and compliance testing. Tensile testing, using a load cell, estimated Young’s modulus (E) of synthetic elastic tubes, allowing c calculation using the Moens–Korteweg equation. Compliance testing under static pressure assessed the relation between P and volume (V). This relationship predicted the compression and contraction courses, validating the testing applied to the lumen of the particular flexible tube. Consequently—to calculate the distensibility of the flexible tube lumen—the change in internal lumen volume due to the change in P was used, hence c corresponding to the Bramwell–Hill equation. Despite limitations such as sample size of tested specimens, experimental conditions not fully physiological, sensitivity of testing due to measurement noise; neglecting anisotropicity and heterogeneity of vessels; not accounting for the full complexity of real arterial behaviour  and the use of synthetic elastic tubes stiffer than in vivo lumens, the calculated c (c_calculated), using the PU-loop technique, was in agreement with values acquired from compliance- and tensile-testing-based calculations. According to compliance and tension testing, the velocity of c was 19.5±0.05 and 19.5±0.012.1 m/s, respectively. Using the PU-loop technique, it was 20±0.25 m/s. These results validate the PU-loop methodology in c assessment.