Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq

Nawres AlKuwaiti

doi:10.1515/agri-2017-0011

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Publication Date

Sun Oct 01 2017

Journal Name

Agriculture (pol'nohospodárstvo)

Volume

63

DOI

10.1515/agri-2017-0011

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Molecular detection of ‘Candidatus Phytoplasma australasia’ and ‘Ca. P. cynodontis’ in Iraq

Nawres AlKuwaiti

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AbstractThe association of phytoplasma was investigated in symptomatic tomato (<italic>Solanum lycopersicum</italic> L.), eggplant (<italic>Solanum melongen</italic> L.), mallow (<italic>Malva</italic> spp.) and Bermuda grass (<italic>Cynodon dactylon</italic> L.) plants exhibiting witches’ broom and white leaf diseases, respectively. Total DNA was extracted from tomato (n=3), eggplant (n=2), mallow (n=2) and Bermuda grass (n=8) samples. Direct polymerase chain reaction (PCR) was performed using P1/P7 primer set, then PCR products were sequenced. Sequences obtained from tomato, eggplant and mallow shared 99% maximum nucleotide identity with phytoplasma belonging to subgroup 16SrII-D, and resulted therefore ‘<italic>Candidatus</italic> Phytoplasma australasia’-related. Sequences obtained from Bermuda grass showed 100% maximum nucleotide identity to 16SrXIV-A subgroup and were ‘<italic>Ca</italic>. P. cynodontis’-related. The study presents the first molecular confirmation and sequence data of presence of ‘<italic>Ca.</italic> P. australasia’ and ‘<italic>Ca</italic>. P. cynodontis’ in Iraq.

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Publication Date

Tue Jul 20 2021

Journal Name

Materials Science Forum

Red Laser Irradiation Effect on the Structural Properties of MawsoniteCu6Fe2SnS8 [CFTS] Thin Films Deposited via Semi-Computerized Spray Pyrolysis Technique

Bahaa T.

Mushtaq Talib

Nathera

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The existing investigation explains the consequence of irradiation of violet laser on the structure properties of MawsoniteCu₆Fe₂SnS₈ [CFTS] thin films. The film was equipped by the utilization of semi-computerized spray pyrolysis technique (SCSPT), it is the first time that this technique is used in the preparation and irradiation using a laser. when the received films were processed by continuous red laser (700 nm) with power (>1000mW) for different laser irradiation time using different number of times a laser scan (0, 6, 9, 12, 15 and 18 times) with total irradiation time (0,30,45,60,75,90 min) respectively at room temperature.. The XRD diffraction gave polycrysta

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