Specimens have been collected from one hundred and seventeen patients residing in local hospitals, 33 with burns and 84 wound injuries,. Three different methods ,Cefoxitin disk diffusion, EPI-M Screening Kit and Crome agar (MeReSa agar)with selective supplement were used to detect methicillin-resistant Staphylococcus aureus (MRSA) . A comparison was made between these 3 methods according to the results. It was found that the results of the Cefoxitin disk diffusion test were compatible with the results of culturing on Crome agar, while those obtained from the EPI-M Screening kit were not accurate and some of them gave false negative results.
Background: there are different procedures for the diagnosis of females suspected with toxoplasmosis. However, time, cost, and accuracy of the test should meet patient’s needs.
Material and methods: one hundred and eleven female with suspected toxoplasmosis were under go three different procedures for the diagnosis of Toxoplasma gondii infection. Latex agglutination test, rapid chromatographic (immunoblot) and ELISA test were done for all patients. The results were described as frequency and percentage of positivity; also, specificity and sensitivity of immunoblot were assessed according to the result of other tests.
Results: The Latex test has shown 80% and 61.54% sensitivity and specificity respectively
Evaluation of Dot. ELISA test for Diagnosis Visceral Leishmaniasis in Infected Children
For the first time in Iraq, this study was conducted to evaluate the usefulness of Dot.ELISA, for detecting anti - Leishmania donovani antibodies in serum samples from suspected patient (children under 8 years ) with Visceral Leishmaniasis V.L.. Sera from 73 V.L. , 60 Healthy controls, and 57 patient with other parasitic diseases other than V.L. (Amoebiasis, Giardiasis , Toxoplasmosis, Schistosomiasis , Hydatidosis, Ascariasis , Lupus Erythromatosus , Viral Hepatitis, and Cutaneous Leishmaniasis) were examined. Anti Leishmania donovani antibodies detected in 71 out of 73 suspected Visceral Leishmaniasis . Data of this study showed that infection in male group was more than female group. Result o
... Show MoreBackground: Helicobacter pylori represents the major etiologic agent of gastritis, gastric and duodenal ulcer disease and can cause gastric cancer. Diagnostic testing for Helicobacter pylori can be divided into invasive and non-invasive techniques based upon the need for endoscopy. Serological test is one of the non – invasive tests although measuring these antibodies is not reliable method of diagnosis but may be used in certain condition.
Objectives: To evaluate serum IgG antibodies against Helicobacter pylori by ELISA technique.
Patients and Methods: The current study consisted of 115 patients (74 males, 41 females) attending The Gastrointestinal tract Center and Gastroscopy department in Baghdad Medical City and was subjected
Background: The diagnosis of Toxoplasma gondii infection in human can be determined by variable immunological and molecular methods.
The objective of this study was to compare the sensitivity and specificity of rK39,
ELISA and IFA tests for the diagnosis of visceral leishmaniasis using blood
samples. Samples were collected from 146 suspected patients and 30 healthy
individual as control. The results showed 52 (35.62%), 58 (39.73%) and 71
(48.64%) positive samples by rK39, ELISA and IFA , respectively. IFA test showed
a higher sensitivity (48.63%) than ELISA and rk39 dip stick (39.72%) and (35.61%)
respectively. The highest sensitivity of agreement (K: 0.912) was found between
rK39 dipstick and ELISA. Therefore, we suggest the application of rK39 dipstick
and ELISA methods in endemic areas of visceral leishmaniasis for early and
accurate diag
Trichomonas vaginalis is an eukaryotic parasite that causes the most common non-viral sexually transmitted infection, trichomoniasis. This disease is responsible for many serious health problems such as preterm birth. More than half of the infected women do not develop symptoms, which makes it difficult to diagnose the
disease. In this study, a specific indirect ELISA method was developed to detect anti-Trichomonas vaginalis IgM and IgG immunoglobulins in the sera of infected females. The aim of this study was to investigate the sensitivity of a simple ELISA procedure in comparison to the classical urine examination and vaginal wet mount preparation for the diagnosis of T. vaginalis. The sensitivity of the indirect ELISA was compared
Fractional calculus has paid much attention in recent years, because it plays an essential role in many fields of science and engineering, where the study of stability theory of fractional differential equations emerges to be very important. In this paper, the stability of fractional order ordinary differential equations will be studied and introduced the backstepping method. The Lyapunov function is easily found by this method. This method also gives a guarantee of stable solutions for the fractional order differential equations. Furthermore it gives asymptotically stable.