In fish, a complex set of mechanisms deal with environmental stresses including hypoxia. In order to probe the hypothesis that hypoxia-induced stress could be manifested in varieties of pathways, a model species, mirror carp (Cyprinus carpio), were chronically exposed to hypoxic condition (dissolved oxygen level: 1.80±0.6mg/l) for 21 days and subsequently allowed to recover under normoxic condition (dissolved oxygen level: 8.2±0.5mg/l) for 7 days. At the end of these exposure periods, an integrated approach was applied to evaluate several endpoints at different levels of biological organisation. These included determination of (i) oxidative damage to DNA in erythrocytes (using modified comet assay), (ii) lipid peroxidation in liver samples by measuring the malondialdehyde production using the 2-thiobarbituric acid [i.e. thiobarbituric acid reactive substances (TBARS) assay] and (iii) histopathological changes in gills. In addition, transcriptional expression of hypoxia-inducible factor 1 α (HIF-1α) and genes involved in the repair of oxidative damage to DNA (i.e. ogg1) and base excision repair (i.e. xrcc1) using reverse transcription polymerase chain reaction in liver samples were also determined. The results suggested significantly enhanced expression of these genes in response to hypoxia compared to concurrent normoxic controls. While the expression of HIF-1α reverted to control values within 7 days exposure to normoxic condition (P < 0.05), the transcriptional expression of the two genes involved in DNA repair process remained significantly high under the recovery period, which complemented the induction of oxidative damage to DNA. Hypoxic groups showed significantly increased values for TBARS level (~2-fold) and histopathological changes in gill tissues compared to both normoxic and recovery groups. Overall, oxidative damage to DNA determined by modified comet assay reflected the observed biological responses in other tissues of the fish. Along with other parameters, this integrated experimental design further strengthens the applications of the comet assay as an important technique to assess stress-induced DNA damage in ecotoxicological studies. © The Author 2014. Published by Oxford University Press on behalf of the UK Environmental Mutagen Society.
Background:
type 2 diabetes mellitus is a chronic disease that is accompanied by the production of free radicals which will ultimately increase the level of oxidative stress.
Hematological parameters were used as a biomarker of sub chronic effect of dursban in Cyprinus carpio L. The common carp was exposed sub chronically to the 36, 53,113 µg /l concentrations of dursban for six weeks. During the experimental period the results showed a decrease in the red blood cell (RBC), white blood cell (WBC), hemoglobin (Hb), mean corpuscular volume (MCV), and packed cell volume (PCV) in the last two weeks of exposure, while the Mean corpuscular hemoglobin (MCH) and Mean corpuscular hemoglobin concentration (MCHC) were significantly increased. The present study indicates that sub chronic exposures of C.carpio to dursban alter the hematological parameters.
The development of thyroid gland in five developmental stages of common carp, Cyprinus carpio L. The thyroid originates from the pharyngeal floor as a median ventral keel in late embryos having four pairs of arches. It differentiates into profollicular aggergations and true follicles with flattened walls and empty lumina or with lumina filled with cotton waste-like material in three days-old elvers. Secretion of homogeneous eosinophilic colloid with a limited increase in the height of follicular wall is noted in five days-old elvers. In nine days-old individuals the follicles increase in size due to coallescence. The thyroid gland reaches its maximal activity as judged by its histology in one-two months-old individuals. The gland is of t
... Show MoreThe present study was conducted to examine toxicological effects of copper sulfate (Cu) in common carp fish (Cyprinus carpio L.). The LC50 (median lethal concentrations) of copper on Cyprinus carpio were 3.64, 3.36, 3.04, 2.65 mg/L respectively. In general, behavioral responses of the fishes exposed to copper included uncontrolled swimming, erratic movements, loss of balance, swam near the water surface with sudden jerky movements. Haematological parameters such, red blood cells (RBC), white blood cells (WBC), haemoglobin (Hb), Packed cell volume (PCV), mean cell volume (MCV) mean cell haemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were studied. The obtained results indicated that the (RBC) and (WBC) have increas
... Show MoreIn present study, an attempt has been made to investigate the effect of Chlorpyrifos insecticide on carp Cyprinus carpio L. through acute toxicity bioassay. The LC50 were estimated as 1897, 1266, 985 and 792 μg/L during 24 , 48, 72 and 96 hr. respectively by using the concentrations 500 , 1000, 1500 , 2000, 2500 and 3000 μg/L indicating high sensitivity of fish to chlorpyrifos. The behavioral response observed in the fish were erratic swimming, loss of reflex, hyperactivities, decrease in appetite secretion of mucus, increase movement of operculum and hyperventilation . These effects increased with increasing concentration of the chlorpyrifos and duration of exposure.
Common carp ( Cyprinus carpio L.) juveniles averaging 7.68± 0.25 - 8.12± 1.14 g in weight were used for 6 weeks to investigate their survival and growth preferences. The experimented carp were stocked at 12 tanks formerly adjusted with different pH levels values as: 6.0, 7.0, 8.0 and 9.0. Growth and survival of common carp was assessed every Sunday of each week. Growth rates significantly (P < 0.05 ) increased at pH 7 and pH8 respectively. Therefore, the results suggest that the water with pH ranged from 7 to 8 was the best range for growth performance and survival rate of carp. Feed conversion ratio (FCR) improved at pH 6 and 9 respectively. In general, the results indicated that water p
... Show MoreThe present study was conducted in order to focus on the effect of the addition of Carnitine and Niacin on some blood serum parameters of Common Carp Cyprinus Carpio. 48 fish carp mean weight 44.13 gm were distributed randomly on four feeding treatments (12 fish each) with replicates (6 fish each) in 8 glass aquaria. Treatments were as follows: fish were fed on basic diet without any addition and conducted as control (T1); addition of 200 mg Carnitine/ Kg diet, (T2) addition of 28 mg Niacin/Kg diet (T3), addition of a mixture of 200 mg Carnitine and 28 mg Niacin/ Kg diet as (T4). The experiment was conducted for 70 days and the results showed an increase in the Cholesterol concentration of T1 (187.6 mg/ 100 ml) and differed s
... Show MoreObjective: To assess nurses' exposure to hospitals chronic diseases hazards in Thi-Qar governorate, and to identify the association between nurses' socio-demographic characteristics of age, sex, marital status, place of work, the experience and educational attainment and their exposure to the hazards of chronic diseases. Methodology: A purposive "non-probability" sample of (433) nurses who were selected from four public hospitals in Thi-qar governorate for the period from November 4th 2013 to June 8th of 2014. Results: The study results indicated that that the vast majority of participants have mild chronic di
This study evaluated the toxicity of ciprofloxacin to spleen and liver when used for the treatment of mice infected with S. typhi for seven days. The dose concentration used in these experiments was 100mg/kg. Mice were divided into two groups . The first group (negative control) was not given ciprofloxacin, but rather a sterile phosphate buffer solution (PBS) as an alternative. Ciprofloxacin was administered to the second group. After seven days , the animals were sacrificed and organs (liver and spleen) were collected . The histopathological examination showed normal hepatocytes in the liver and normal structure of spleen cells in animals of control group . However, the treated group showed dilated and congested blo
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