The current study was conducted to evaluate the effect a mixture of threespecies of arbuscular mycorrhizal fungi (Glomus etunicatum, G. leptotichum andRhizophagus intraradices) double and triple mixture and organic matter by usingplastic pots in the greenhouse at some mycorrhiza and physiological limitationscharacteristics in tomato plant after four and eight weeks of cultivation. Theresults of the determinants mycorrhiza significant increase the percentage ofmycorrhizal frequency F% dry weight of roots mycorrhiza (g.plant-1) andorganic matter in all mycorrhiza single, double and triple mixture after four andeight weeks cultivation treatments. The highest percentage of mycorrhizalfrequency and increase the dry weight of the root in the treatment of the mixturemycorrhiza triple and organic matter compared with the other treatments aswell as the results after eight weeks cultivation higher than four weeks. Thestudy showed that the total shoot and root dry weight and organic matterpresence a significant increase in the total dry weight of shoot and root in singletreatments and double and triple mixture and organic matter after four andeight weeks cultivation compared with the control treatment, and appeared thehigher total dry weight of shoot of the plant in the treatment of the triplemycorrhiza mixture compared with other treatments and eight weeks afterplanting higher than four weeks Agriculture. Also caused all single, double andtriple treatments mycorrhiza mixture and organic matter after eight weeks ofAgriculture is also a significant increase in the elements nitrogen, phosphorusand potassium, as well as protein in the roots of tomato plants were treated triplemixture of all the elements and protein is higher than other treatments.