This paper presents a comparison study on thermal performance conic cut twist tape inserts in laminar flow of nanofluids through a constant heat fluxed tube. Three tape configurations, namely, quadrant cut twisted tape (QCT), parabolic half cut twisted tape (PCT), and triangular cut twisted (VCT) of twist ratio= 2.93 and cut depth= 0.5 cm were used with 1% and 2% volume concentration of SiO2/water and TiO2/water nanofluids. Typical twist tape with twist ratio of= 2.93 was used for comparison. The results show that the heat transfer was enhanced by increasing of Reynolds number and nanoparticles concentration of nanofluid. The results have also revealed that the use of twist tape enhanced the heat transfer coefficient significantly and maximum heat transfer enhancement was achieved by the presence of triangular cut twist tape insert with 2% volume concentration of SiO2nanofluid. Over the range investigated, the maximum thermal performance factor of 5.13 is found with the simultaneous use of the SiO2nanofluid at 2% volume concentration VCT at Reynolds number of 220. Furthermore, new empirical correlations for Nusselt number, friction factor, and thermal performance factor are developed and reported.