Isolation and identification of polyhydroxyalkanoates producing bacteria from biopolymers waste in soil

Ithar Kamil Al-Mayaly Al-Mayaly

doi:10.1088/1757-899X/928/6/062014

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Publication Date

Sun Nov 01 2020

Journal Name

Iop Conference Series: Materials Science And Engineering

Volume

928

DOI

10.1088/1757-899X/928/6/062014

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Isolation and identification of polyhydroxyalkanoates producing bacteria from biopolymers waste in soil

Ithar Kamil Al-Mayaly Al-Mayaly

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Abstract<p>The production of polyhydroxyalkanoates PHAs from biopolymer degrading bacteria was examined <italic>in situ</italic> by screening isolates using Sudan B Black staining process as potential PHAs detecting, and Nile Blue staining as a proof method detection. Five bacterial strains isolated from biopolymer waste buried in a garden soil were able to produce high rate of PHA. <italic>AK1P</italic> and <italic>AK2P</italic> strains demonstrated high productivity of biopolymer by converting 5% (w/v) lactose as the only carbon source to PHA during fermentation. <italic>AY2P</italic> strain converted 5% (w/v) of glucose with less PHA accumulation. The favorite temperature for those strains to produce a high rate of PHA was at 30° C.</p>

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