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CLINICAL UTILITY OF URINARY ANTIGEN TEST AND MOLECULAR METHOD FOR DETECTION OF LEGIONELLA PNEUMOPHILA

Background: Legionella pneumophila (L. pneumophila) is gram-negative bacterium, which causes Legionnaires’ disease as well as Pontiac fever. Objective: To determine the frequency of Legionella pneumophila in pneumonic patients, to determine the clinical utility of diagnosing Legionella pneumonia by urinary antigen testing (LPUAT) in terms of sensitivity and specificity, to compares the results obtained from patients by urinary antigen test with q Real Time PCR (RT PCR) using serum samples and to determine the frequency of serogroup 1 and other serogroups of L. pneumophila. Methods: A total of 100 pneumonic patients (community acquired pneumonia) were enrolled in this study during a period between October 2016 to April 2017; 92 samples were collected from patients attended and admitted to Al-Imamein Al-Kadhimein Medical City and 8 samples from those in the (Center of Kidney Diseases and Transplantation) in the Medical City of Baghdad. All patients were under therapy with antibiotics. Serum and urine specimens were obtained from all patients; urine samples were processed for urinary antigen test (rapid test). Serum samples were collected and submitted to DNA extraction for detection of L. pneumophila mip gene by q RT PCR assay. Results: The percentage of L. pneumophila in two hospitals in Bagdad was 30%. Of these 26% was serogroup 1 detected by urinary antigen testing (UAT). In the other hand, 23% of samples were positive by q RT PCR based mip gene, of these 19 % were serogroup 1 and 4% were another serogroup. The sensitivity of UAT is high (P value < 0.001), which means statistically highly significance than q RT PCR. Conclusion: LPUAT is a rapid tool for early diagnosis of Legionella infection, which highlights the need of using this test in hospitals and health institutions and there is a high prevalence of L. pneumophila in Iraq that refer to the necessity of considering this microorganism point of view in future studies for detection and treatment in pneumonic patients. Keywords: L. pneumophila, mip gene, quantitative real time PCR, urinary antigen. Citation: Gauad SA, Abdulrahman TR, Muhamad AK, Jawad AA, Hassan JS. Clinical utility of urinary antigen test and molecular method for detection of Legionella pneumophila. Iraqi JMS. 2018; 16(2): 207-215. doi: 10.22578/IJMS.16.2.13

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Publication Date
Fri Jun 16 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
The Isolation and Identification of Salmosamonella typhi from clinical samples with molecular detection of O-antigen encoded genes

Abstract

Antibiotic treatment of  S.typhi is difficult as compared to treatment of acute infection. Antibiotic resistance carried against S.typhi  by using 6 kinds of antibiotics from  different classes, their results  showed that all isolates were  high resistance to Ampicillin (99%), Gentamicin (98%), Amikacin (79%) and   less  resistances  Trimethoprim (55%) ,  Imipenem (60%)  and Ceftriaxone(66%)  .

The present study focused on the molecular detection of Wzx flippase, Wzy polymerase genes in some Salmonella typhi isolates, Samples were collected from typhoid patients by classical lab work. Antibiotics susceptibilit

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Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
Detection of Adenovirus antigen and its correlation with clinical signs among children with gastroenteritis

A total of 90 stool sample was collected from patients with gastroenteritis to
detect adenovirus antigen among diarrhea cases. They were tested by general stool
examination (GSE), rapid immunochromatographic test and Enzyme Linked
Immunosorbent Assay (ELISA). GSE showed that adenovirus gastroenteritis
infection resulted in non-bloody diarrhea, the existence of RBCs in 7% and Pus in
37% of the samples, Entamoeba histolytica trophozoite and cyst were seen in 3%
and 2% of the samples respectively. The rapid test showed that 21% of samples
were positive for rotavirus, 8% for adenovirus and 3% for astrovirus. Meanwhile,
the ELISA test showed that adenovirus was positive in 9% of the samples. These
findings establish

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Publication Date
Sat Apr 15 2023
Journal Name
Iraqi Journal Of Science
Detection of Respiratory Syncytial Virus infection in Clinical Samples Using Immunofluorescence Test

Current study aimed to investigate the Respiratory Syncytial Virus (RSV) in nasal
secretion and throat swab samples of hospitalized patients with symptoms of
respiratory tract infection using Immunofluorescence test. Previously these samples
were tested for Respiratory Syncytial Virus infection by reverse transcriptase-PCR
assay. The positive samples were tested by Immunofluorescence assay in monolayer
confluent of Hep 2 cell line. The results showed that the positive samples using the
RT-PCR test were positive in this test. These results reveal that Immunofluorescence
test is sensitive method for detection the infection with RSV.

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Publication Date
Sun Jul 01 2007
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The clinical utility of serum uric acid measurement in pre-eclampsia

Background:To evaluate the accuracy of serum uric acid to predict maternal and fetal complications in women with pre- eclampsia.
Design: Prospective study. Setting: Tikrit Teaching Hospital.
Subject and Methods: Fifty normotensive and forty-three pre-eclamptic pregnant women at (20-24) weeks of gestation were the study subject, serum concentrations of uric acid
were measured and examine the correlations between serum uric acid level with fetal and maternal complications.
Result: Serum concentration of uric acid in pre-eclamptic women were significantly higher than in gestational age match normotensive pregnant women, and women with pre-eclampsia
and elevated uric acid concentration at increased risk of

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Publication Date
Sat Dec 31 2022
Journal Name
Al-kindy College Medical Journal
Molecular Microbiology in Clinical Practice: Current and Future Applications: Molecular Microbiology

Technological advances have yielded new molecular biology-based methods for the diagnosis of infectious diseases.  The newest and most powerful molecular diagnostic tests are available at regional and national reference laboratories, as well as at specialized centers that are certified to conduct metagenomic testing.  Metagenomic assays utilize advances in DNA extraction technology, DNA sequence library construction, high throughput DNA sequencing and automated data analysis to identify millions of individual strands of DNA extracted from clinical samples.  At present, metagenomic assays are only possible at a small number of special research, academic and commercial laboratories.  Continued research in human and path

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Publication Date
Sun Jan 03 2016
Journal Name
Journal Of Educational And Psychological Researches
Test the effectiveness of Method EMDR

Our country faced lots of crises specially Wars and still living under the traumatic events. This would result in psychological disorder specially the Acute Stress Disorder (ASD). That’s if not treated, it will turn to be over Post Traumatic Stress Disorder(PTSD). Also not mentioning the shortage of recourses speaks about war and crises. That treat with its inflections psychologically and sociologically theses cases if happened.

The importance of this study arise through it is objective to introduce a program for EMDR which give benefit for treat in health, social, educational institutes.

Aims:

The objective of this Study is the identification of a Test the effectiveness of Eye Movement Desensi

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Gel clot assay used for detection of candida spp onfection in urinary tract

the study including isolation and identification of candida spp causing UTIs from patintes coming to al-yarmouk hospital

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Publication Date
Sun Apr 03 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Detection of Galactomannan Antigen in the Serum of Iraqi Patients with Suspected Invasive Aspergillosis

Invasive aspergillosis is a severe infection that occurs in patients with prolonged neutropenia, following chemotherapy,transplantation,or immunosuppressive protocols .Galactomannan ( GM) is a molecule ,found in the cell wall of Aspergillus species and is released in the blood during growth .The detection of GM in the blood is used to diagnose the invasive Aspergillosis in humans using ELISA assay.
Objectives: To detect Galactomannan antigen in in the serum of immunocompramized patients suspected to have invasive aspergillosis.
Patients and methods: This study was conducted on 50 patients from the hematology&oncology department,of Baghdad teaching hospital and pediatric oncology wards ,from March 2013 to October 2013.The patien

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Publication Date
Wed Oct 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Serological and molecular detection of parvovirus b19 in children with acute lymphoblastic leukemia.

Background: Human parvovirus B19 (B19V) is the only human pathogenic parvovirus, It is highly erythrotropic and preferentially replicates in erythroid progenitor cells (EPCs). Recently the effects of B19 infection have been well studied in children with acute lymphoblastic leukemia (ALL).

Objective: To detect parvovirus B19 among children with ALL.

Methods: A cross sectional study involved forty five patients with ALL (21 patients were newly diagnosed ALL and 24 children who underwent chemotherapy) who were attending department of oncology in Children's Welfare Teaching Hospital in Baghdad Medical City between December 2012 and April 2013, compared to forty five of appare

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Publication Date
Mon Dec 28 2020
Journal Name
The Iraqi Journal Of Veterinary Medicine
Serological and Molecular Phylogenetic Detection of Coxiella burnetii in Lactating Cows, Iraq

This study is carried out to investigate the prevalence of Coxiella burnetii (C. burnetii) infections in cattle using an enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) assay targeting IS1111A transposase gene. A total of 130 lactating cows were randomly selected from different areas in Wasit province, Iraq and subjected to blood and milk sampling during the period extended between November 2018 and May 2019. ELISA and PCR tests revealed that 16.15% and 10% of the animals studied were respectively positive. Significant correlations (P<0.05) were detected between the positive results and clinical data. Two positive PCR products were analyzed phylogenetically, named as C. burnetii IQ-No.5 and C. burnet

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