Objectives: The purpose of this in vitro study was to compare the effect of adding a poloxamer surfactant to the irrigant solutions on its cleaning efficiency. Design: In this study the roots of extracted permanent premolar teeth were used and evaluated by using Scanning Electronic Microscopy (SEM). Materials and Method: 72 human single tooth of permanent premolar (8 for each group) were used in this in vitro study. Roots after sectioning at cervical area to get 15 mm were embedded in a plastic container filled with impression silicon, then instrumented with ProTaper rotary instruments till size F4. Each group (8 root) were irrigated with one of the nine solutions used in study: three concentrations of NaOH [5% (A1), 2.5%(A2), 0.5%(A3)], three concentrations of NaOH with Poloxamer surfactant [5% (B1), 2.5% (B2), 0.5% (B3)], NaOCl 5.25% (C1), NaOCl 5.25% with Poloxamer surfactant (C2), and Normal Saline (D). After instrumentation, each tooth was finally washed with normal saline. Then by using diamond disc, all roots were sectioned longitudinally in buccolingual direction to obtain two halves of each root, the more intact root was selected to be prepared for scanning electron microscopy test. Finally, the data was analyzed by using the SPSS 25 program with a non-parametric Kruskal-Wallis test. Additionally, scores were analyzed by using the chi-square test. The significance level for all tests was set at p ≤ 0.05. Results: the study showed that Sodium hypochlorite was higher in smear layer removal than that of Sodium hydroxide, and there was also a significant increase in the cleaning efficiency of sodium hydroxide with increasing concentration of solutions. Conclusion: The adding of a poloxamer surfactant didn't significantly increased the cleaning efficiency of both solutions used. No significant difference between NaOCl and NaOH