A joke is something that is said, written, or done to cause amusement or laughter. It could be a short piece or a long narrative joke, but either way it ends in a punchline, where the joke contains a second conflicting meaning. Sometimes when we read a joke, we understand it directly and fully, but this is not always the case. When a writer writes a joke, he intends to manipulate the reader in a way that the reader doesn’t get the joke at once. He does that by using pun on words or any other word play. We, as listeners to the joke, try to get the message depending mostly on the tone of the voice, in addition to other factors concerning vocabulary and grammar. But as readers of the joke, we need more other factors in order to get to the intended meaning of the joke. One of these important factors is punctuation. Punctuation is the use of certain signs which help understand a piece of writing. It is used to create clarity, sense, and stress in contexts, because using the correct punctuation helps us to convey our thoughts as we intend them to. So is the use of punctuation marks in writing a joke is essential in order to understand it? Or is it just helpful sometimes? What happens if the writer doesn’t use punctuation marks when writing the joke? Would this affect us in getting the meaning of the joke? In this study we try to answer these rising questions.