Knowledge and awareness towards cryptosporidiosis were assessed in this study during the period from April 2009 till June 2011, among 188 person of both gender which were divided in different groups and sub groups as following Group 1: include 48 physician from different hospitals and primary health care centers in Baghdad this group were sub divided into 30 Specialist doctors and 18 General practitioners doctors. Group 2 : include 45 teaching staff member from Biology department , Biotechnology department in University of Baghdad and AL-Nahrain University as well as teaching staff member from the college of medicine – University of Baghdad and University of Mustansiryah, this group were sub divided into 9 Ph.D and 36 M.Sc. + B.Sc. member and Group 3: include 95 students from Biology department – University of Baghdad this group were sub divided into13 postgraduate students and 82 undergraduate students group from the last year in the college. Interview method was depended on a questioner made of three parts (general knowledge , mode of transmission and epidemiology + life cycle) . Results showed that the awareness was moderate towards cryptosporidiosis in the three groups of this study the mean of scores were (66.54 ± 14.69) towards all items of questioner, the mean of scores obtained in general knowledge part (72.39 ± 20.26) were higher than mean of scores obtained in both mode of transmission (63.93 ± 18.57) and epidemiology + life cycle part(63.29 ± 18.69) , gender are non significant factor affected in awareness towards cryptosporidiosis as well as there were no significant grade differences among the three groups in this study.