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This research was conducted to measure the safety of heat stable enterotoxin a (STa) produced by
enterotoxigenic Escherichia coli, through studying its toxic effect on human blood lymphocyte, since it showed a
promising effect in reducing the proliferation of colorectal cancer cells. the cytogenetic effects of (STa) by using
five different concentrations (100, 200, 400, 800 and 1600μg/ml) in comparison with negative (PBS, Phosphate
buffer saline) and positive (MMC, Mitomycin C) at concentration of 5μg/ml, controls on human blood
lymphocytes obtained from both (10) normal healthy persons and (20) colorectal cancer patients was measured
by employing the following parameters: mitotic index, blast index, chromosomal aberrations and micronucle
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