ANTIBACTERIAL AND CYTOTOXIC EFFECTS OF THE KIWI FRUIT AND POMEGRANATE ACTIVE COMPOUNDS ON TUMOR CELL LINE (L20B, RD)

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20/10/2014 12:24 PM

ANTIBACTERIAL AND CYTOTOXIC EFFECTS OF THE KIWI FRUIT AND POMEGRANATE ACTIVE COMPOUNDS ON TUMOR CELL LINE (L20B, RD)


 
Ebtehal H. Al-Naimy
Raghad K. Al-Lihaibi
Suad M. Majeed
Rafal S. Al-Ani
Biotechnology Research Center- University of Al-Nahrain


ABSTRACT


This study was designed to evaluate the anti bacterial activity and the cytotoxic effect of Actinidia deliciosa ( Kiwi fruit ) and Punica granatur (pomegranate). The chemical detection was made for the plants extract and revealed that the plants extract were positive for flavonoids and phenols. Also the antibacterial activity for both plants extract was done in different bacterial species include gram positive bacteria ( Staphylococcus aureus , Bacillus cereus ) and gram negative bacteria ( Escherichia coli , Vibrio cholerae ) using agar diffusion and well diffusion methods. Also both plants extract cause an inhibitory effect on both gram negative and gram positive bacteria. The highest antibacterial activity was recorded against S. aureus for both extracts and the inhibition zone reached 23mm in a well diffusion method for pomegranate extract and 19mm in a diameter for Kiwi fruit extract. The cytotoxic effect of both plants extract was carried out on two tumor cell line ( L20B and RD ).The result showed that the plants extract cause a significant differences (P < 0.05) between means of cell viability of both L20B and RD cultures, also both plants extract showed an inhibitory effect on L20B and RD cell line growth rate after 72 hr. of incubation for all concentrations used to reached maximum significant decrease at concentration 1000 μg/ml in comparison with the control



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