EFFECT OF NITROGLYCERIN ON IN VITRO MATURATION OF SHEEP OOCYTES

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20/10/2014 10:24 AM

EFFECT OF NITROGLYCERIN ON IN VITRO MATURATION OF SHEEP OOCYTES


 
Muhammad- Baqir M-R. Fakhrildin / Institute of Embryo Research and Infertility Treatment - Al-Nahrain University
Ali A. I. AL Juaifri
Abdulla M.H. Shubber
Dept. Animal Resources - Coll. of Agric. - Univ. of Baghdad


ABSTRACT


This Study was conducted to investigate the effects of different concentrations of nitroglycerine (NTG) (0, 0.05, 0.1, 0.15, 0.3 AND 0.5 μM) supplied with two types of culture media (Roswell Park Memorial Institute – 1640; RPMI- 1640 and simple medium for assisted reproductive technology; SMART on in vitro maturation (IVM) of sheep oocytes. This study was executed in the laboratories of the Institute of Embryo Research and Infertility Treatment / AL- Nahrain University during the period from December, 2009 to June, 2010. The ovine ovaries were used as a source of oocytes. oocytes were collected using aspiration technique. One thousand and three hundred and twenty four oocytes were collected from 844 ovaries obtained from local abattoir. Most of recovered collected oocytes were immature (1139 oocytes). A significant ( p< 0.05) increase in IVM oocytes with using 0.05 and 0.1 μM of NTG supplied with RPMI – 1640 as compared with control and other treated groups . On the other hand, using of SMART in comparison with 0.05μ M NTG lead to an obvious (p < 0.05) increases in IVM oocytes as compared with the control and remaining treated groups. In conclusion, using 0.05 and 0.1μM of NTG within RPMI-1640 medium produces improvement in the percentages of IVM as well as Enrichment 0.05μM of NTG to SMART medium produced increases percentages of IVM, while supplemented 0.5 μM of NTG to RPMI-1640 and SMART media produced decreases percentages of IVMP



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