The Effect of 532nm Nd: YAG Pulsed Laser on the Activity of Superoxide dismutase and Alcoholdehydrogenase of Saccharomyces Cerevisiae

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15/7/2014 10:16 AM

The Effect of 532nm Nd: YAG Pulsed Laser on the Activity of Superoxide dismutase and Alcoholdehydrogenase of Saccharomyces Cerevisiae



Noor A. Jadah
Ayad G. Anwer
Institute of Laser for Postgraduate Studies, University of Baghdad, Baghdad, Iraq.
Ghazi M. Aziz / Department of Biotechnology, College Of Science, University of Baghdad, Baghdad, Iraq.
(Received 5 January 2010; accepted 2 May 2011)


Abstract :


In this study, the effect of Nd: YAG laser on the activity of superoxide dismutase (SOD) and alcoholdehydrogenase (ADH) was investigated. The Saccharomyces cells were irradiated using 532nm Q-Switched Nd: YAG laser with (1Hz) frequency. Different fluences 11.3, 22.6 and 33.9mJ/cm2 and different number of pulses 15, 30 and 60 pulse were used. The irradiated cells were incubated in a liquid nutritive medium for 24 hours. After incubation, the cells were harvested and disrupted to extract the intracellular enzymes and their activities were assessed. In comparison with the control, the irradiated cells showed a significant increase in the activity and the specific activity of SOD at energy densities of 11.3 and 22.6mJ/cm2 at 30 and 60 pulses respectively. Maximum activity and specific activity of ADH was observed using 11.3mJ/cm2 and 22.6mJ/cm2 at 15 pulses. While an inhibition in both activity and specific activity of two enzymes was observed at 33.9 mJ/cm2. It is concluded that the fluence at 22.6mJ/cm2 stimulate the activity and specific activity of the two enzymes to a maximum values.




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