Inhibition Activity of Thuja occidentalis Extract Against Staphylococcus aureus and Candida albicans Isolated form cases of Skin Infections.

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12/5/2014 10:36 AM

Inhibition Activity of Thuja occidentalis Extract Against Staphylococcus aureus and Candida albicans Isolated form cases of Skin Infections.


Bushra. J. Mohammed
Genetic Engineering and Biotechnology Institute University of Baghdad


Abstract

The study includes isolation and identification of Staphylococcus aureus and Candida albicans form cases of skin infections and evaluation the inhibitory effect of Thuja occidentalis extract by measurement the inhibition zone and Phenol coefficient. The identification of bacteria and fungi was carried out by morphological, cultural and biochemical tests.
Forty seven isolates of S. aureus , 31 isolates of C. albicans and 26 isolates of mixed infection were recovered from 104 positive swaps taken from cases of skin infections. All isolates were submitted to sensitivity testes to antibiotic and it was found that S. aureus isolates exhibited complete resistance to Ampicillin, Tetracycline, Gentamicin, Rifampicin, Ceftriaxon, Cefalexin, Trimethoprim, Streptomycin and Neomycin. While C. albicans isolates exhibited complete resistance to Miconazol, Clotrimazole and Ketoconazole. The results of phenol coefficient revealed that the effect of the Thuja occidentalis extracts have higher effect on S. aureus and C. albicans in comparison with phenol. The outcomes of measurement the inhibition zone indicated height antimicrobial activity of Thuja occidentalis extract reached to 29 and 38 mm against S. aureus and 23 and 31 mm against C. albicans at concentration 40 and 50% respectively.
Key word: Thuja occidentalis, C. albicans, S. aureus, phenol coefficient.



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