Production and Partial Purification Of Protease By Fusarium spp. By Solid State Fermentation.

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08/5/2014 10:39 AM

Production and Partial Purification Of Protease By Fusarium spp. By Solid State Fermentation.



 Ayat Adnan Abbas Hussain
Biotechnology Research Center AL-Nahrain University


Abstract

In this paper, A strain οf Fusarium spp., is grown on potato dextrose agar and on wheat bran Czapek Dox medium. It was found to produce neutral protease. Fructose, when added to the culture medium was found to give rise to the neutral protease produced in the presence of fructose and was also found to enhance the production of neutral protease. production was carried out by solid state fermentation The fungi were grown on czepek- Dox with wheat bran culture medium. The optimum conditions were determined as follows, dilution ratio 1:30, temperature 25° C, pH 7.0, incubation time 9 days, inoculums size 1 × 106 ( spore /ml), carbon source and nitrogen source were fructose ,ammonium nitrate respectively. The protease was isolated from the culture filtrates and was DEAE- (diethylaminoethyl) DEAE-cellose. The specific activity of pure enzyme was 30.211 u/mg, with No. of fold 8.945 and yield 0.188%, gradient buffer treatment,and characterization of pure enzyme optimum pH ,pH stability 6- 11 and 11 respectivly. Optimum temperature and stability temperature 40° C and 20° C and had a Vmax of 2 of protein and Km value of 1.5mg/ml towards casein and 3.9 km ,3 vmax of BSA.



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